Concept
Heterotrophic prokaryotes with relatively high nucleic acid
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200010/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | HetHNA | |
| Definition | Heterotrophic prokaryotes include both Bacteria and Archaea. When they do not contain any photosynthetic pigments and thus do not have any auto-fluorescence property exploitable by flow cytometry, they require staining with a fluorescent dye. In most studies a nucleic acid dye is used. Most of the time, staining of nucleic acids by a green emitting fluorescent dye is used. Blue laser excitation enables heterotrophic prokaryotes to be distinguished in various groups thanks to sideward or forward scatter, and green fluorescence signatures: cells with a higher green fluorescence signal correspond to heterotrophic prokaryotes with a relatively high nucleic acid content (HetHNA). Their scatter signals (forward and sideward) are equal or lower than those of OraPicoProk and RedPicoEuk, and may overlap those of RedPicoProk. | |
| Date | 2022-11-13T22:13: | |
| Identifier | SDN:F02::F0200010 | |
| Note | accepted | |
| Has Current Version | 4 | |
| Version | 1 , 2 , 3 | |
| version | 4 | |
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