Concept
Orange fluorescing microphytoplankton
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200016/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | OraMicro | |
| Definition | The OraMicro group consist of cells greater than 20 µm They display higher forward scatter and orange fluorescence signals than OraNano, and a higher orange fluorescence/red fluorescence ratio compared to the RedMicro group. Staining is not required to distinguish them by flow cytometry. Cells have a high phycoerythrin content and may also contain phycocyanin that can be excited by a red laser. In this case, when cells are excited by a laser beam with a wavelength approximately of 533 nm (green), then they will emit a higher red fluorescence signal compared to chlorophyll a-only containing cells due to the energy transfer to the pigment phycocyanin that will also emit fluorescence in the red fluorescence channel. This group can include cells or groups of cells greater than 20 µm including cryptophytes, colonial cyanobacterial filaments, but also dinoflagellates and some ciliates. Due to the small volumes usually analyzed by flow cytometry (typically less than 1 cm^3), this group is not always properly quantified when cells are not abundant enough. When forward scatter is calibrated using solutions of beads with different diameters or phytoplankton cell-cultures, it is possible to distinguish microphytoplankton from nanophytoplankton cells with a size close to 20 µm. Chains or colonies may be too large for flow cytometry analysis, depending on instrument characteristics (tubing inner diameter, flow cell dimensions, pulse shape recording or not). | |
| Date | 2022-11-13T22:13: | |
| Identifier | SDN:F02::F0200016 | |
| Note | accepted | |
| Has Current Version | 3 | |
| Version | 1 , 2 | |
| version | 3 | |
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