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Concept

filtration, DNA extraction, PicoGreen staining, primer selection

URI http://vocab.nerc.ac.uk/collection/S03/current/S03410/
Within Vocab BODC parameter semantic model sample preparation entity descriptions
Alternative Labels
Definition Separation of suspended solids from the sample, which then has its DNA content extracted, labelled with PicoGreen for quantification and processing to target specific subcomponents (genes) of the DNA molecules
Date 2010-01-11T11:21:54
Identifier SDN:S03::S03410
Note accepted
Has Current Version 1
version 1
Broader S01:S019 sample preparation method entity Mapping: 1471969
Related
P01 BODC Parameter Usage Vocabulary - (6) [+]
P01:Z4124515 Abundance of Cyanobacteria (ITIS: 601: WoRMS 146537) [Subcomponent: nifH gene copies Subgroup: filamentous phylotype] per unit volume of the water body by filtration, DNA extraction, PicoGreen staining, primer selection and quantitative PCR Mapping: 1237051
P01:L0302763 Abundance of Cyanobacteria (ITIS: 601: WoRMS 146537) [Subcomponent: nifH gene copies Subgroup: unicellular group A phylotype] per unit volume of the water body by filtration, DNA extraction, PicoGreen staining, primer selection and quantitative PCR Mapping: 1237063
P01:D1453672 Abundance of Gammaproteobacteria (WoRMS 393018) [Subcomponent: nifH gene copies Subgroup: gamma A phylotype] per unit volume of the water body by filtration, DNA extraction, PicoGreen staining, primer selection and quantitative PCR Mapping: 1237075
P01:X27B9BA9 Abundance standard deviation of Cyanobacteria (ITIS: 601: WoRMS 146537) [Subcomponent: nifH gene copies Subgroup: filamentous phylotype] per unit volume of the water body by filtration, DNA extraction, PicoGreen staining, primer selection and quantitative PCR Mapping: 1237087
P01:TAB9993A Abundance standard deviation of Cyanobacteria (ITIS: 601: WoRMS 146537) [Subcomponent: nifH gene copies Subgroup: unicellular group A phylotype] per unit volume of the water body by filtration, DNA extraction, PicoGreen staining, primer selection and quantitative PCR Mapping: 1237101
P01:AA844163 Abundance standard deviation of Gammaproteobacteria (WoRMS 393018) [Subcomponent: nifH gene copies Subgroup: gamma A phylotype] per unit volume of the water body by filtration, DNA extraction, PicoGreen staining, primer selection and quantitative PCR Mapping: 1237115

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